High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring.CRISPR/Cas systems have emerged as transformative tools in biosensing due keychron m4 to their unparalleled specificity, programmability, and unique enzymatic activities.They exhibit two key cleavage behaviors: precise ON-target cleavage guided by specific protospacers, which ensures accurate target recognition, and bystander cleavage activity triggered upon target binding, which enables robust signal amplification.
These properties make CRISPR/Cas systems highly versatile for designing biosensors for ultra-sensitive detection.This review comprehensively explores recent advancements in CRISPR/Cas system-based biosensors, highlighting their impact on improving biosensing performance.We discuss the integration of CRISPR/Cas systems with diverse signal readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), and so on.
Additionally, we examine the development of integrated biosensing systems, such as microfluidic devices and portable biosensors, which leverage CRISPR/Cas technology for point-of-care testing (POCT) and high-throughput analysis.Furthermore, we identify unresolved challenges, aiming to inspire music hall mmf-1.3 turntable innovative solutions and accelerate the translation of these technologies into practical applications for diagnostics, food, and environment safety.